KMID : 0624620090420120840
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BMB Reports 2009 Volume.42 No. 12 p.840 ~ p.845
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Casein Kinase 2 interacts with human mitogen- and stress-activated protein kinase MSK1 and phosphorylates it at Multiple sites
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Shi Yan
Wu Huiling Ye Kan Tian Zhipeng Wang Jiaqi Shi Huili Huo Keke Han Guanghui Ye Mingliang Zou Hanfa
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Abstract
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Mitogen- and stress-activated protein kinase (MSK1) palys a crucial role in the regulation of transcription downstream of extracellular-signal-regulated kinase1/2 (ERK1/2) and mitogen- activated protein kinase p38. MSK1 can be phosphorylated and activated in cells by both ERK1/2 and p38¥á. In this study, Casein Kinase 2 (CK2) was identified as a binding and regulatory partner for MSK1. Using the yeast two-hybrid system, MSK1 was found to interact with the CK2¥â regulatory subunit of CK2. Interactions between MSK1 and the CK2¥á catalytic subunit and CK2¥â subunit were demonstrated in vitro and in vivo. We further found that CK2¥á can only interact with the C-terminal kinase domain of MSK1. Using site-directed mutagenesis assay and mass spectrometry, we identified five sites in the MSK1 C-terminus that could be phosphorylated by CK2 in vitro: Ser757, Ser758, Ser759, Ser760 and Thr793. Of these, Ser757, Ser759, Ser760 and Thr793 were previously unknown.
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KEYWORD
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CK2, Mass spectrometry, MSK1, Phosphorylation, Yeast two-hybrid
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